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polarstar omega  (BMG Labtech)


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    Structured Review

    BMG Labtech polarstar omega
    Polarstar Omega, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 97/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polarstar+omega/SPECTROstar+Omega/pmc12858634-489-41-49
    Average 97 stars, based on 489 article reviews
    polarstar omega - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Biomimetic Silk Nanoparticle Manufacture: Calcium Ion-Mediated Assembly.
    Article Snippet: .. After a 10 min incubation at ambient temperature, the bound thioflavin T fluorescence intensity was read (excitation = 440 nm, emission = 475 nm) (Polarstar Omega, BMG Labtech, Ortenberg, Germany). ..

    Fluorescence:

    Article Title: Biomimetic Silk Nanoparticle Manufacture: Calcium Ion-Mediated Assembly.
    Article Snippet: .. After a 10 min incubation at ambient temperature, the bound thioflavin T fluorescence intensity was read (excitation = 440 nm, emission = 475 nm) (Polarstar Omega, BMG Labtech, Ortenberg, Germany). ..

    Article Title: Oxidized Cholesterol Derivatives in Fraction B Prepared from Gulf Catfish ( Arius bilineatus , Val.) Skin Regulate Calcium Response and Neutrophil Extracellular Traps (NETs) Formation
    Article Snippet: .. Following the addition of different compounds (S1 to S14) and cells activation, changes in fluorescence intensities were measured every 30 min for up to 210 min using a POLARstar OMEGA (BMG Labtech, Ortenberg, Germany) with excitation at 504 nm and emission at 523 nm. ..

    Bioluminescence Resonance Energy Transfer:

    Article Title: Screening assays, modulators and modulation of activation of Receptor for Advanced Glycation End-products (RAGE)
    Article Snippet: Bioluminescence Resonance Energy Transfer (BRET) BRET is an established technology for studying protein-protein proximity in live cells, particularly involving GPCRs (Pfleger and Eidne, 2006). .. Plasmids were transiently co-expressed in human embryonic kidney (HEK) 293FT cells and BRET measurements taken at 37° C. using a POLARstar Omega or LUMIstar plate reader (BMG Labtech, Mornington, Victoria, Australia) with 460-490 nm (‘donor emission’) and 520-550 nm (‘acceptor emission’) filters, or a VICTOR Light plate reader (Perkin Elmer, Glen Waverley, Victoria, Australia) with 400-475 nm (‘donor emission’) and 520-540 nm (‘acceptor emission’) filters, or a CLARIOstar plate reader (BMG Labtech, Mornington, Victoria, Australia) with 420-480 nm (‘donor emission’) and 520-620 nm (‘acceptor emission’) filters. ..

    other:

    Article Title: Protocol for detecting endogenous GPCR activity in primary cell cultures using ONE-GO biosensors
    Article Snippet: POLARstar Omega , BMG Labtech , N/A.

    Article Title: Protocol for detecting endogenous GPCR activity in primary cell cultures using ONE-GO biosensors.
    Article Snippet: Janicot & Garcia-Marcos, STAR Protocols 5, 103355 December 20, 2024 a 2024 ONE vector G protein Optical (ONE-GO) biosensors can measure the activity of endogenously expressed G protein-coupled receptors (GPCRs) in primary cells.. By detecting G proteins that belong to all four families (Gs, Gi/o, Gq/11, G12/13) across cell types, these biosensors provide high experimental versatility.. We first describe steps to express ONE-GO biosensors in primary cells using lentiviral transduction.

    Activation Assay:

    Article Title: Oxidized Cholesterol Derivatives in Fraction B Prepared from Gulf Catfish ( Arius bilineatus , Val.) Skin Regulate Calcium Response and Neutrophil Extracellular Traps (NETs) Formation
    Article Snippet: .. Following the addition of different compounds (S1 to S14) and cells activation, changes in fluorescence intensities were measured every 30 min for up to 210 min using a POLARstar OMEGA (BMG Labtech, Ortenberg, Germany) with excitation at 504 nm and emission at 523 nm. ..

    Centrifugation:

    Article Title: Functional characterisation of Target of Rapamycin (TOR) signalling in Physcomitrella
    Article Snippet: After mixing for 5 min at Vmax using an Eppendorf ThermoMixer, samples were centrifuged at 20,000 × g for 10 min at 2 °C and 400 μL of supernatants were collected. .. After an additional round of centrifugation for 5 min, three technical replicates of 100 μL of each supernatant were loaded on a 96-well microplate (Greiner, PS, F-Bottom, Crystal-Clear) and absorbance was read at 645 nm and at 663 nm using a POLARstar Omega or a SPECTROstar Nano microplate reader (BMG LABTECH, Ortenberg, Germany). ..

    Luciferase:

    Article Title: Inactivated cGAS‐STING Signaling Facilitates Endocrine Resistance by Forming a Positive Feedback Loop with AKT Kinase in ER+HER2– Breast Cancer
    Article Snippet: Briefly, at 12 h post transfection, the cells were pretreated with the indicated inhibitors for 2 h, then 2 μg mL −1 HT‐DNA were transfected into cells and lysed in a passive lysis buffer (Promega) 16 h after HT‐DNA transfection. .. The luciferase assays were performed using a dual luciferase assay kit (Promega), quantified with POLARstar Omega (BMG Labtech), and normalized to the internal Renilla‐luciferase activity. ..

    Activity Assay:

    Article Title: Inactivated cGAS‐STING Signaling Facilitates Endocrine Resistance by Forming a Positive Feedback Loop with AKT Kinase in ER+HER2– Breast Cancer
    Article Snippet: Briefly, at 12 h post transfection, the cells were pretreated with the indicated inhibitors for 2 h, then 2 μg mL −1 HT‐DNA were transfected into cells and lysed in a passive lysis buffer (Promega) 16 h after HT‐DNA transfection. .. The luciferase assays were performed using a dual luciferase assay kit (Promega), quantified with POLARstar Omega (BMG Labtech), and normalized to the internal Renilla‐luciferase activity. ..



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